Mosharraf Sarker is a human biologist whose research interests lie in the understanding of mammalian cell signalling mechanism to inflammation and oxidative stress. Currently he is also working on understanding the beneficial interaction of probiotic bacteria and health. He obtained his undergraduate and Master of Science degrees in Physiology from the Institute of Postgraduate Medicine and Research, Dhaka, Bangladesh (1982-1987).
Dr. Sarker obtained his PhD in vascular physiology from King’s College London under the supervision of Dr. Paul Fraser (1991-1995). The subject of his thesis was the mechanisms by which some inflammatory mediators increase cerebral microvascular permeability. Following this he undertook two postdoctoral research fellowships, firstly working with Dr Fraser and then with Dr. Fraser and Dr. Kevin Pedley at the Vascular Biology Research Centre, Cardiovascular Division, King’s College London. Both Fellowships was funded by the Welcome Trust where Dr. Sarker was the named applicant. Dr. Sarker is still collaborating with Dr. Fraser at King’s College London as a visiting lecturer. Dr. Sarker was also taught at the Pharmacy Department, Jahangirnagar University, Dhaka, Bangladesh.
Dr. Sarker took up a lectureship in human biology at Teesside University in 2001. At Teesside, Dr. Sarker has developed a tissue culture facility to study the role of probiotic bacteria to inflammation and oxidative stress. Currently a number of PhD students, MSc and undergraduate students are working in his lab in related fields.
Since 2007 Dr. Sarker has developed a link with the internationally renowned vascular biology research group at the Institute of Ophthalmology, University College London (UCL) and Cellular and Molecular Imaging Laboratory, Henry Ford Hospital, Detroit, USA through which he has been able to spend time doing research in UCL and in the USA as an academic visitor. Dr. Sarker is currently appointed as Honorary Lecturer in the Institute of ophthalmology at UCL, London (Oct 2013-Sep 2018).
Dr. Sarker has so far successfully supervised one PhD student to completion as director of studies and is currently supervising a number of PhD students. Dr. Sarker has also taken an active role in research management within the School of Science & Engineering and the University as Postgraduate Tutor (2007-to date); Chair of the University Research Degree Sub-committee (Oct 2913 –to date); member of the School Research Degree Committee (2004-2013) and University Research Degree Committee (2007-to date) and also was Vice Chair of the School Research Degrees Committee (SRDC) (2007-2009). He was an active member of the School Research Ethics Committee (2003-2010). Dr. Sarker also led the undergraduate final year research project as a module leader (2003-2007) and was a course leader for MRes in Science (2008-2011).
1. Microscopy: Fluorescence microscopy specially to measure intracellular calcium, free radicals and nitric production in live animal model in vivo and also in vitro cultured cell systems.
2. Cell culture: both primary and cell lines; permeability studies both in vitro and in vivo.
3. Molecular technique to detect protein expression, gene expression.
4. Fluorescence microscopy and gentamicin protection assay (GPA) to study Phagocytic activity of immune cells.
Role of Probiotics in the modulation of bacterial macrophage interaction
This project is designed to study role of probiotic bacterial supernatant to phagocytic activity of murine macrophagic cell line. The ability of probiotics to influence immune function is still poorly understood. This study utilised a gentamicin protection assay (GPA) to assess the influence of probiotics on the interaction of E. coli with the murine macrophage cell line J774. Findings will help us in understanding the effect of probiotics on bacterial ingestion.
Probiotic mediated anti-inflammatory and anticancer cell signalling in cultured cell system
In this project we have been studying the effect of probiotic bacteria in the regulation of inflammatory, antioxidant, cancer proteins expression in macrophage and in a number of colon cancer cells.
Dr. Sarker would welcome enquires from companies which require expertise related to investigating any product (natural/synthetic) for their health beneficial effects.
Paul Canning, Bridget-Ann Kenny, Vivien Prise, Josephine Glenn, Mosharraf H. Sarker, Natalie Hudson, Martin Brandt, Francisco J. Lopez, David Gale, Philip J. Luthert, Peter Adamsonf, Patric Turowski, and Alan W. Stitt, (2016) Lipoprotein-associated phosphoplipase A2 (Lp-PLA2) as a therapeutic target to prevent retinal vasopermeability during diabetes. PNAS, Vol. 113(26), 7213-7218.
Y. S. Nanjundaiah, D. A. Wright, A Baydoun, Z. Ali, L.T. O’Hare, Z. Khaled, & M. Sarker (2016) Lactobacillus rhamnosus GG conditioned media modulates acute reactive oxygen species and nitric oxide in J774 murine macrophages, Biochemistry and Biophysics Reports. Vol.6, 68-75.
Hudson, N., Powner M.B. Sarker M.H., Burgoyne T., Campbell M., Ockrim Z.K., Martinelli, R.S., Futter, C.E., Grant, M.B., Fraser, P.A., Greenwood, J., Shima, D.T. and Turowski, P. (2014) Differential apicobasal VEGF Signaling at Vascular Blood-Neural Barriers. Developmental cell, Vol. 30, 541-552.
MA Patwary, KA Rahman, MH Sarker (2014) Layered cake clientelism: Impact on occupational safety in organizational management. Journal of Scientific Research and Reports. Vol. 3(16), 16-31.
MA Patwary Lucina QU, WTO, MH Sarker (2014) Social Ostracism and Criminality among Medical Waste Workers in Bangladesh: Neurobiological Aspects. Journal of Scientific Research and Report, Vol. 3(11), 1475-1489.
Nnamdi EJ and Sarker M (2013) Fluorescence investigation of discriminatory phagocytosis of live Escherichia coli and Lactobacillus rhamnosus by J774 Murine macrophage. E3 Journal of Biotechnology and Pharmaceutical Research. Vol. 4(4), pp. 61-67.
Masum A Patwary, Lucina Q. Uddin, M. Abdul Jalil Miah, Mosharraf H. Sarker (2013) Occupational stress and addiction: possible neurobiological elucidation of medical waste related individuals. Journal of Hospital Administration, 2013, Vol. 2 (2): 1-18.
Patwary MA, O’Hare WT, Karim SA, Sarker MH. (2012) The motivations of young people moving into medical waste scavenging as a street career. Journal of Youth Studies. 15(5): 591-604.
Patwary, M.A. and Sarker, M.H., 2012. Quantitative assessment of mortuary waste: occupational safety and environmental health. Journal of Hospital Administration, 1(1), 49-60.
Patwary, M. A., O'Hare, W. T. and Sarker, M. H. (2012) 'Occupational accident: An example of fatalistic beliefs among medical waste workers in Bangladesh', Safety Science. Vol. 50(1), pp. 76-82.
Patwary, M. A., O'Hare, W. T. and Sarker, M. H. (2011) An illicit economy: Scavenging and recycling of medical waste. Journal of Environmental Management, Vol. 92 (11), 1-7, 2011.
Patwary, M. A., O’Hare W.T. and Sarker M.H. (2011) Assessment of occupational and environmental safety associated with medical waste disposal in developing countries: a qualitative approach. Safety Science, 49, vol. 49(8-9) pp.1200-1207.
Woodfin, A. et. al. (2011) Acute NADPH oxidase activation potentiates cerebrovascular permeability response to bradykinin in ischemia–reperfusion. Free Radical Biology and Medicine, 50 (4), pp.518-524.
Patwary, M. A., O’Hare W.T. Elahi K.M. Hassan, M.M., Sarker M.H. (2010) 'Domes and the Dead: An example of extreme fatalism among mortuary workers in Bangladesh', Kaleidoscope: The Interdisciplinary Postgraduate Journal of Durham University’s Institute of Advanced Study; Afterlives, 4 (1), pp.10-18. (Cited:7)
Sarker, M. H., Hu, D.-E. and Fraser, P. A. (2010) Regulation of cerebromicrovascular permeability by lysophosphatidic acid. Microcirculation, 17 (1), pp.39-46.
Patwary,M.A, O’Hare, W.T., Street,G., Elahi, K.M., Hossain, S.S., Sarker, M.H. (2009) Quantitative assessment of medical waste generation in the capital city of Bangladesh. Waste Management, vol. 29 (8), 2392–2397.
Patwary, M A, O’Hare, W.T., Street, G., Elahi, K.M., Hossain, S.S., Sarker, M.H. (2009) 'Health and safety perspective on medical waste management in a developing country: A case study of Dhaka city', 19th international conference on flexible automation and intelligent manufacturing, University of Teesside, July 6-8 2009, in Nabhani, F. (ed) Proceedings of the 19th international FAIM conference. Gemini International Limited, pp.282-290.
Victoria Gauden, D.-E. Hu, T. Kurokawa, M.H. Sarker & P.A. Fraser (2007) Novel technique for estimating cerebrovascular permeability demonstrates capsazepine protection following ischemia-reperfusion. Microcirculation, 4(8), 767-78.
Sarker M.H., and Fraser P.A. (2002) The role of guanylyl cyclases in the permeability response to inflammatory mediators in pial venular capillaries in the rat. J Physiol. 540: 209-218. (Cited:34)
Sarker M.H., Hu D.-E. and Fraser P.A. (2000) Acute effects of bradykinin on cerebral microvascular permeability in the anaesthetized rat. J. Physiol. 528.1:PP.177-187. If 4.38
Sarker M.H., Easton A.S. and Fraser P.A. (1998) Regulation of cerebral microvascular permeability by histamine in the anaesthetized rat. J. Physiol. 507.3:PP.909-918. (Cited:36)
Easton A.S., Sarker M.H., and Fraser P.A. (1997) Two components of blood-brain-barrier disruption in the rat. J. Physiol. 503.3: PP.613-623. (Cited: 28)
Rashid HU. Khan AH. Sarker MH. Akhter S. Khan MF (1996) Effect of haemodialysis on serum zinc, copper and magnesium levels in patients with end stage renal failure. Bangladesh Renal Journal. Vol 15(1): pp 15_17. (Cited:1)
Wolff C B, Sarker M. H. and Fraser P.A. (1995) Cerebral microvascular permeability and CSF pH in anaesthetized rats. Experimental Physiology, 80: 1053-1055. (cited:2)
Huq F., Islam M.A., Sarker M.H., Chowdhury B., Ali M.W. and Kabir M.M. (1995) Epidemiology of snake bite in Bangladesh. Bangladesh J. Zool, Vol.23 (1):61-64. (Cited:8)
Roy J., Khan I.H., Dutta A.K. Sarker M.H., Choudhuri S.K., and Mahmud M. (1992) Traditional herbal therapy using a mixture of Allium Sativa (garlic) and Nigella Sativa (Kalajira) in the treatment of Urinary tract infections. Bangladesh Renal Journal, Vol. 11(1): 13-17. (Cited:2)
Sarker M.H., Hai M.A., and Khanum F.A. (1988) Study of blood pressure in relation with physical activity. Bangladesh Renal J., vol. 7(2): 57-60.
Sarker M.H., Chowdhury R.I., Mohibulla M., and Sultana S. (1989) Effect of age, sex, and body weight on blood pressure in the urban population of Dhaka. J. of IPGMR, vol. 4(2): 48-52.
(My current h-index is 10 -only 17 publications are available in the Scopus; rest can be found in Google scholar)