Introduction to upstream bioprocessing

Duration: 3 days
Price: £2,250+VAT

Our introductory courses are aimed at scientists, operatives and managers who are at an early stage in their career, and who want to gain a broad understanding of the strategies and techniques used in biomanufacturing. Delegates are introduced to industry standard procedures, their underlying principles and how these procedures are applied within the fields of biotherapeutics, vaccines and advanced therapy manufacturing.

The creation of this course has been funded by IUK through the ATSTN.

  • UKRI
  • atstn

    Training partners

  • Cytiva
  • Chemometec

Delivered in partnership with industry experts the Introduction to Upstream Bioprocessing training course introduces delegates to common concepts and terminology as used in upstream bioprocessing within a modern biomanufacturing facility. Delegates will learn about the principles of cell culture including adherent versus suspension cell culture, bioreactor design and operation, single-use upstream technologies, process control and evaluation, and scale-up strategies. Our unique training facility houses state-of-the-art laboratory and bench-scale processing equipment that provides delegates with the opportunity to gain hands-on practical training in typical biomanufacturing processes and procedures.

Module 1
• Cell culture to bioreactor
• Expression systems and cell requirements
• Cell counting methods, validation, and variation
• Introduction to WAVE and Xcellerex bioreactors
• Lab activity – Cell Thaw
• Lab activity – Subculturing
• Lab activity – Aseptic techniques

Module 2
• Process evaluation, data recording and handling
• Process control in bioreactors - PID control loops and process automation
• Industry case study - Process development framework
• Lab activity – hands-on with Wave, part 1
• Lab activity – hands-on with XDR-10, part 1
• Lab activity – manual and automated cell counting

Module 3
• Adherent cell cultivation
• Scale-up and tech transfer
• Concepts of process optimisation
• Lab activity – hands-on with Wave, part 2
• Lab activity – hands-on with XDR-10, part 2
• Virtual reality activity - 15L pilot scale fermenter

Learning outcomes:

  • Develop an understanding of recombinant cell expression and cell requirements in upstream culture
  • Describe the design principles of cell culture systems and apply your understanding to evaluate alternative cell culturing systems for the production of biotherapeutic products
  • Learn and apply fundamental process control principles and bioreactor design to common upstream scenarios
  • Evaluate manual and automated cell imaging techniques for total cell count and cell viability

Who should attend:

  • Early career biotechnology professionals with existing preliminary knowledge of; entry level cell biology, the basics of recombinant expression and upstream technologies, general laboratory techniques (including media preparation and subculture of adherent/suspension cells) and fundamentals of bioreactor use.
  • Individuals would typically be qualified with a relevant further education qualification such as a diploma or BSc in Science. Attendees without the recommended background or qualifications will be offered additional ‘access to the course’ support.

Course dates

Tuesday 14 - Thursday 16 February 2023

For enquiries and details of the full agenda please email CPDNHC@tees.ac.uk

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