Duration: 3 days
Price: £2,250+VAT
This course is aimed at research and process development scientists and other advanced therapy professionals wishing to deepen their understanding of flow cytometry and fluorescence-activated cell sorting (FACS). Delegates are introduced to industry standard procedures, their underlying principles and how these procedures are applied within the field of advanced therapy research, development, and manufacturing.
Delivered in partnership with our strategic partner Beckman Coulter this three-day course combines lectures and hands-on practical sessions to introduce flow cytometry for the characterisation of advanced therapeutic products. Lecture topics include flow cytometry basics, fluorescence compensation, cellular assays, protein quantification, and cell sorting. Through laboratory activities delegates will experience the state-of-the-art equipment here at the National Horizons Centre including a Beckman Coulter Moflo Astrios EQ Cell Sorter for cell analysis and cell sorting plus a Beckman Coulter CytoFLEX cytometer suitable for cell analysis within a regulated environment due to its 21 CFR Part 11 compliant software. Delegates will also be introduced to emerging technologies in the field of at-patient, flow cytometry.
Upon completion of the course, delegates will have a deep understand and experience of state-of-the-art flow cytometry techniques and methodologies and appreciate the current and future challenges in applying this technology to advanced therapies biomanufacturing.
Module 1 - Flow cytometry basics
• Principle of flow cytometry, system components and how it works
• Applications of flow cytometry in cell and gene therapy
• Flow cytometry with multi-colour fluorescence and compensation to minimize spectral overlaps
• Familiarisation and operation of flow cytometer instruments
• Manual and automated multi-colour fluorescence compensation
Module 2 - Cellular and DNA content analysis
• Measurement of viable and apoptotic cell populations with flow cytometry
• Measurement of cell cycle and ploidy with flow cytometry
• Measurement of cell proliferation by CFSE dye dilution
• Gating strategies and effective flow cytometry data analysis
• Staining cells with propidium iodide for cell cycle and Annexin V for apoptosis
• Running the stained cells on the flow cytometer for cell cycle and apoptosis analysis
Module 3 - Protein detection and cell sorting
• Protein detection and immunophenotyping with antibodies on flow cytometry
• Multiplex cytometric bead array for quantifying multiple proteins simultaneously
• Automated flow cytometry system for at-patient cell characterisation.
• Principle of fluorescence-activated cell sorting (FACS)
• Instrument calibration for sorting, and hands-on FACS sorting into tubes
• Hands-on FACS sorting into 96-well plates
Learning outcomes:
Who should attend:
Tuesday 16 – Thursday 18 May 2023
For enquiries and details of the full agenda please email CPDNHC@tees.ac.uk
