Advanced downstream bioprocessing

Duration: 5 days
Price: £3,750+VAT (academic discounts may be available, please enquire)

Our advanced courses are aimed at those working in research and development, process development and those are who are already working in the sector, who want to gain a deeper understanding of the theory and techniques used in biomanufacturing, understand and experience state-of-the-art techniques and methodologies, and appreciate the current and future challenges in biomanufacturing.

The creation of this course has been funded by IUK through the ATSTN.

  • UKRI
  • atstn

    Training partners

  • Cytiva
  • Merck
  • Sartorius
  • Watson Marlow
  • Tecan
  • Repligen

Delivered in partnership with industry leaders this five-day course provides in-depth training in downstream bioprocess operations as carried out in a modern biomanufacturing facility, with an emphasis on biotherapeutics, vaccines and advanced therapies. Typical process flows will be presented, and topics include design of experiments, harvest strategies, ultrafiltration, diafiltration, viral clearance and chromatography. In addition, industry experts will introduce advances in product recovery and purification technologies with the opportunity for delegates to discuss case studies and applications. Our unique training facility houses state-of-the-art laboratory and bench-scale processing equipment that provides delegates with the opportunity to gain hands-on practical training in typical biomanufacturing procedures.

Module 1
o Clarification
o Flocculation
o Lab activity – CHO Cell Harvest
o Lab activity – Yeast Cell Harvest
o Lab activity – Fill-Finish & Fluid Transfer

Module 2
o Integrated Viral Clearance
o Workshop activities
o Hardware & Software Tools for Downstream Process Development
o Unicorn System Control
o Unicorn Method Editor
o Chromatogram Evaluation

Module 3
o Essentials of Protein Purification
o Capture Chromatography
o HIC, Multimodal & Special Capture Resins
o AKTA Automation and Design of Experiments
o Periodic Counter-current Chromatography
o Lab activity – Akta Chromatography System
o Lab activity – Column Packing

Module 4
o Considerations for TFF Operation
o Lab activity - Concentration and Diafiltration of clarified BSA and Vit B12, part 1
o Process Development for TFF
o Lab activity - Concentration and Diafiltration of clarified BSA and Vit B12, part 2
o Lab activity – Normalised Water Permeability
o Lab activity - TFF Data Analysis

Module 5
o Lab automation and RoboColumns
o Workshop – DSP Design Activity

Learning outcomes:

  • Gain practical and industry focused knowledge of harvest principles and techniques.
  • Develop an in depth understanding of chromatography and resin formats and apply this understanding to hands-on scenarios.
  • Advance your knowledge of crossflow filtration processes through hands-on operation, demonstration and analysis of data received from an example process.
  • Evaluate advanced technologies and applications including periodic counter-current chromatography and viral clearance strategies.
  • Apply the advances in DSP to the challenges of scale up, GMP compliance and automation.

Who should attend:

  • Scientists, technical staff, engineers, and managers with at least 3 years’ experience within the biotechnology industry wishing to increase their knowledge of downstream operations in a modern bioprocessing plant, through lectures and hands-on training.

Course dates

More dates coming soon

For enquiries and details of the full agenda please email CPDNHC@tees.ac.uk

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